Coding

Part:BBa_K2557048:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


PhiC31-RDF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 911
    Illegal BamHI site found at 997
    Illegal BamHI site found at 1244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
    Illegal NgoMIV site found at 307
    Illegal NgoMIV site found at 349
    Illegal NgoMIV site found at 1920
    Illegal AgeI site found at 6
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 603
    Illegal BsaI.rc site found at 1006
    Illegal BsaI.rc site found at 1359
    Illegal BsaI.rc site found at 2001
    Illegal SapI.rc site found at 2603


Design Notes

We have optimized the codons.


Source

We found the sequence of PhiC31-RDF from the literature and synthesized this sequence.

References